| 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid HEPES |
| Alias: |
N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid |
| CAS NO.: |
7365-45-9 |
| Molecular formula: |
C8H18N2O4S |
| Structure: |
 |
| Specifications: |
| Sepcification |
Standard |
| Appearance |
White Crystalline Powder |
| Assay % |
≥99.5 |
| Solubility(1m Aq) |
Clear,colorless solution |
| PH(1% aq) |
5.0-6.5 |
| Heavy Metals (PPM) |
≤10 |
| Loss On Drying % |
≤1.0 |
| UV 260nm(1m aq) |
≤0.1 |
| UV 280nm(1m aq) |
≤0.07 |
| Fe Iron ppm |
≤5 |
| Sulfate Ion % |
≤0.05 |
|
| Purpose: |
4- hydroxyethyl piperazine ethyl sulfonic acid is a biological buffer; The reaction of the separation and analysis of RNA component buffer, buffer, hybridization buffer in advance; Used for RNA and T4RNA ligase 3'- terminal marking; Used in biochemical diagnostic kit, DNA/RNA extraction kit and PCR diagnostic kits. To uninfected cells function. It is a kind of hydrogen ion buffer, can control the scope of the pH value of the constant for a long time. Use the final concentration of 10-50 tendency for L, general culture medium containing 20 tendency LHEPES buffering capacity could be achieved. |